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文献和实验SSR GEL and Silver Staining Protocol
as above, rinsed with d.i. water and stored in fresh fixer without agitation. 4. Rinse the gel 3 times for 2 minutes each with distilled water with gentle agitation. 5. Add staining solution to gel and gently agitate for 30 minutes, decant and precipitate silver
Silver Enhancement of Colloidal Gold
separately in a freezer. Incubation time for 1-nm gold is about 20 min, for Nanogold about 25 min (20 - 22°C). Silver lactate (Lah et al., 1990) As above, replace citrate buffer by 0.2 M HEPES buffer, pH 6.8. Incubation time ~3 min
Size Measurement of Nanoparticles Using Atomic Force Microscopy
are provided. The nanoparticles cited are National Institute of Standards and Technology (NIST) Au nanoparticle Reference Materials RM 8011 (nominally 10 nm particles), RM 8012 (nominally 30 nm), and RM 8013 (nominally 60 nm).
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