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文献和实验Mag-Binds Plant DNA Kit Magnetic Fast Protocol
with appropriate steel beads. Add 600ul Buffer SLX Minus and 4ul RNase A. 2. Process in the mixer mill machine by following manufacturer’s instructions. Time and speed will need to be determined for each type of sample. 3. Incubate at 70°C for 10
Mag-Binds Plant DNA Kit Magnetic 96 Protocol
SLX Minus. 2. Incubate at 70°C for 10 minutes. Mix sample once during incubation shaking. 3. Centrifuge at 3,000-5,000 x g (5,000 x g is better, if available) for 10 min. Compact pellets will form at bottom of tubes but some particles
Dynabeads® Co-Immunoprecipitation Kit
. Place the tube on a magnet, allow the beads to collect at the tube wall. Remove the supernatant. 10. Short SB Wash: Add 0.8 or 1.6 ml of SB and mix by vortexing or pipetting. Place the tube on a magnet, allow the beads to collect at the tube
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