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产品简介
Fast T4 DNA Ligase 来源于重组大肠杆菌菌株,可以高效催化双链 DNA 或 RNA 上相邻的 5´ -磷 酸末端和 3´ -羟基末端形成磷酸二酯键。本品不仅能够催化平末端或粘性末端之间的连接,还可以修复双链 DNA、RNA 或 DNA/RNA 杂交双链中的单链缺口。
产品应用
1. NGS 建库中,双链 DNA 与接头连接
2. DNA 片段与载体的连接
3. 双链 DNA、RNA 或 DNA/RNA 杂交体中的缺口修复
4. 线性双链 DNA 环化
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文献和实验Ligation of DNA with T4 DNA Ligase
recombination and DNA synthesis. DNA ligase from E. coli is a polypeptide with a molecular weight of 74,000 and is NAD-dependent. T4 DNA ligase is the product of gene 30 of the T4 phage, has a molecular weight of 68,000, and is ATP-dependent. Both enzymes
Joining RNA Molecules with T4 DNA Ligase
Researchers interested in studying RNA structure and function or RNA-protein interactions are increasingly using site-specifically modified RNAs to probe sites of interest (1 ). In addition to expanding the repertoire of functional groups
Splint Ligation of RNA with T4 DNA Ligase
Splint ligation of RNA, whereby specific RNA molecules are ligated together, can be carried out using T4 DNA ligase and a bridging DNA oligonucleotide complementary to the RNAs. This method takes advantage of the property of T4 DNA ligase
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