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文献和实验Live imaging with Drosophila tissue culture cells
) (see note 3): Culture dishes or slides with coverslip bottom are used. Some providers produce round culture dishes (e.g. Willco Wells B.V.®) or 8-well or single well culture slides (e.g. Nunc™ or ibidi®, see figure 2); To follow
Dorsal Root Ganglion Neuron Culture from Adult Rats
Description Procedure for culturing and maintaining Dorsal Root Ganglion (DRG) neurons from adult rats. Procedure Preparing Culture Surface - Collagen coat cover slips (if using) or cell culture dishes: evenly spread 2-3 drops of rat
E.Z.N.A.® Total RNA Midi Kit Protocol for Eukaryotic Cells and Tissues
min at 5000 x g to completely dry the HiBind® matrix. 7. Elution of RNA. Transfer the column to a clean 15 ml centrifuge tube (Not supplied) and elute the RNA with 250-500ul of DEPC-treated water (supplied with kit). Make sure to add water
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