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文献和实验Chromatin Assembly Using Drosophila Systems
, Potter‐Elvehjem homogenizer) 500‐ml centrifuge bottles for Sorvall GSA rotor (or equivalent) Mira cloth (Calbiochem
CSF Extract Prep for Spindle Assembly
that we find are most important for obtaining good CSF extracts that are competent for CSF spindle assembly and for cycled spindle assembly. The indicated buffer amounts are sufficient for a 4 frog prep. For sperm nucleus preparation, see above protocol. For spindle assembly
Assembly of Nucleosomal Templates by Salt Dialysis
., Walker, K.P., and Hansen, J. 1999. Assembly of defined nucleosomal and chromatin arrays from pure components. Methods Enzymol. 304:19‐35. Hayes, J.J. and Lee, K.M. 1997
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