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文献和实验+ 0.1% SDS (500 mls) 50 mls 20X 5 mls 10% SDS= 0.1% SDS Running buffer (1L): 100 mls 10X MOPS 52.6 mls of formaldehyde 847.4 mls H20 RNA Gel (70ml): 0.84 g agarose 7 mls 10x MOPS 58.38 mls H2O put gel in solution by heating let cool to 60°C
Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
("phagemid") between the initiation site ("I") and the termination site ("T") within the central portion of the bacteriophage lambda between the two "arms" of the phage genome that are crucial for its function. Before screening, the expression library
activity 3000 Ci/mmol; Amersham Pharmacia Biotech) Drosophila Schneider SL2 tissue culture cells or other tissue or cell line for analysis, with appropriate growth facilities and equipment Ethanol (100% and 70%) Ethidium bromide (10 mg
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