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文献和实验Sample Preparation for Microinjection
. Therefore, the filled capillary should be inserted into the capillary holder and immediately lowered into the medium to avoid blockage due to drying. The capillary should always remain in the medium especially during long experiments when large numbers of cells
RNA AND PROTEIN EXTRACTION FROM THE SAME SAMPLE
and pour into a 15 mL centrifuge tube. Depending on the kind of tissue, extra extraction buffer and b -mercaptoethanol may be required NOTE: for protein analysis: remove ~0.5-1.0 mL into eppendorf tube containing 1-5 m L of 0.1M PMSF. Mix
AML Sample genomic DNA Preparation
and transfer 0.9 mL of cells to a 1.5 mL tube. The remainder of cells should be placed in a control rate freezing jar and placed at -80ºC. Transfer back to the original freezer boxes at -80ºC. Make a note in the MPD Frozen Samples that 1/2 of the sample
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