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文献和实验. F. Biomek-automated modified-Eperon isolation procedure for single-stranded M13 DNA This semi-automated method is a modification of a previously reported procedure (24,25), and allowed the simultaneous isolation of 48 single-stranded
ArrayPlex SA: A Turn-Key Automated Gene Expression Target Preparation System
, on the fully integrated ArrayPlex SA� system, based on the field proven Biomek FX�, which streamlined the target preparation procedure allowing up to 96 samples to be processed in less than 36 h. The process is comprised of three functional methods, cDNA
Bst-catalyzed radiolabeled DNA sequencing
. Each extension reaction contained 500-750ng of Biomek isolated single-stranded DNA, reaction buffer, nucleotide extension mix, oligonucleotide primer (typically M13 (-40) universal sequencing primer, see Appendix D), either [a-32-P]dATP or [a-35-S
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