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文献和实验Obtaining Embryos: hCG Injection
Ovulation is induced in X. tropicalis by injection of human chorionic gonadotropin (hCG). X. tropicalis requires a much smaller dose than does X. laevis. We use basically the same protocol as that described on the Grainger site .
Earlier studies from our laboratory have demonstrated the appearance of a high Mr (182-kDa) phosphoprotein during early stages of development of cardiac hypertrophy in the sera of animals subjected to aortic constriction. Furthermore
Intracytoplasmic Spermatocyte Injection (ICSI)（图）
, Nagy Z, Joris H, Liu J, Staessen C, Smitz J, Wisanto A, Devroey P. (1993) High fertilization and implantation rates after intracytoplasmic sperm injection. Hum Reprod 8, 1055-1060. We would like to thank Dr. S. Al Hasani, Clinic for Gyniatrics
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