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文献和实验Kingfisher Flex 96 Plant DNA High Pure Protocol
Solution and 500ul Lysis Buffer to the sample. 7. Set up KingFisher Flex instrument by press “tart Key”on the Mag Bind Plant DNA Protocol and load plates according to prompts from Kingfisher Unit. 8. After the DNA isolation, seal Elution Plate
Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC
on the monitor). 2. Wash out unbound axonemes with 80 µl of BRB80 + 1 mM DTT + 1 mM GTP + 0.5 mg/ml HSA 3. Flow in at least 3-4 chamber volumes of tubulin mix (i.e. tubulin in BRB80 + 1 mM DTT + 1 mM GTP + 0.5 mg/ml HSA), seal edges with Valap
High‐Throughput Cytotoxicity Screening by Propidium Iodide Staining
., Young, S.M., Oprea, T.I., Bologa, C., Prossnitz, E., and Sklar, L.A. 2006. Biomolecular screening of formylpeptide receptor ligands with a sensitive, quantitative, high‐throughput flow cytometry platform. Nat. Protoc. 1:59‐66
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