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文献和实验DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
. In general, the methyl green slide is photographed at a higher power than the H&E. The pathologist then reviews the methyl green photo along side of the H&E slide. The methyl green photo is then marked by the pathologist to define the areas
samples from the thermal cycler and immediately place on ice, with an ice pack on top of the samples, for 1 min. Turn off the power supply. Adjust comb if it has been pushed out of the gel during pre-heating. Load 4 µl of each sample per well. TIP
Western Blotting Using the SemiPhor Semi-Dry Transfer Unit
to a suitable power supply. Turn the power supply to zero before turning on. Turn on the power supply and set to approximately 0.8 mA/cm2 of gel. 8 mm x 7 mm mini-gels may be run at 100 mA constant current. Larger gels must be limited to 0.8 mA/cm2 to avoid
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