- ￥44000
- Beckman Coulter贝克曼库尔特
- 美国
- C63138
- 2025年07月12日
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文献和实验Overview This is a method to assemble two BioBricks using the Clontech In-Fusion PCR Cloning Kit and maintains BioBrick standard formats. There are currently several BioBrick assembly standardsthat all involve assembly by restriction enzyme
Chromatin Assembly Using Drosophila Systems
15‐ml Wheaton dounce homogenizer, “A” pestle Sorvall Superspeed centrifuge with SS‐34 rotor (or equivalent)
CSF Extract Prep for Spindle Assembly
Spin at #5 for 30 sec and full speed for 15 sec in a clinical centrifuge. Remove all buffer and versilube (as dry as possible) Crush @ 16 deg C: 15 min @ 10,000 rpm (full brake) in an SW55 rotor. We find that using the ultracentrifuge
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