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- Beckman Coulter贝克曼库尔特
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- 2025年07月11日
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文献和实验Protocol for vacuum-infiltration transformation of Arabidopsis thaliana
and 5% sucrose ).Batches of 100 to 500 plants, 3 to 4 weeks old and well developped, were taken out of the soil, rinsed with water, and immersed in 2 l of Agrobacterium -containing IM medium in a vacuum chamber (10 l volume). Plants were put under vacuum (10^4 Pa
Green lab protocol for vacuum infiltration transformation of Arabidopsis
stoppers sitting in the culture. Make sure no large bubbles are trapped under the plant. 6. Draw a vacuum of 400 mm Hg (about 17 inches). Once this level has been obtained, close the suction (i.e., so that the vacuum chamber is still under 17 inches
so that a 75 x 25 x 1 mm microscope glass slide will sit flush in the chamber. 7) Create a recess for an O-ring around the underside of the top and bottom plates such that they are spaced apart by 0.02". Insert the rubber O-rings. 8)
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