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文献和实验SSR GEL and Silver Staining Protocol
method is simpler than the other methods. 1. Remove clamps. Clean excess polyacrylamide and urea from the top of plate assembly with d.i. water. Pull the comb out of the mold slowly and evenly, cleaning out the comb area with d.i.water or buffer. 2. Add
SSR GEL and Silver Staining Protocol
of SSRP buffer heat 95o C for 2 min., ice and 5 ul 1X PE II buffer 2.5 ul of 400 ng / ul of sonicated Poly-dA 7.5 ul SSRP buffer VI. ELECTROPHORESIS: 1. Remove clamps. Clean excess polyacrylamide and urea from the top of plate assembly with d
with a license for target validation. A typical 0.2 µmol-scale RNA synthesis provides about 1 milligram of RNA, which is sufficient for 1000 transfection experiments using a 24-well tissue culture plate format. It should be noted that the use of siRNAs
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