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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
selector to 50 mF 5. Set Load resistance to 200W 6. Turn on power supply 7. Set maximum power to 25 W 8. Set current to 25 mA 9. Set voltage to 1800 V 10. Allow power supply to stabilize (still in pulse position) 11. Select
2-D Polyacrylamide Gel Electrophoresis
to the anode (Red/+)and the acidic side closer to the cathode (Black/-)to be consistent. 12.Run gels at 40 mA/gel for 15 min or until dye front is about an inch into the gel. 13.Turn down voltage and run at 65-70 V constant overnight.(If in a hurry,set power
Pyrocarbonate (DEPC) treated H2O Master set of clone-purified, sequence verified human ESTs (e.g. gf211 release, Research Genetics, Huntsville, AL) 96 pin inoculating block (#VP 4088, V&P Scientific, Inc, San Diego, CA) Airpore Tape Sheets, (# 19571
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