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- Beckman Coulter贝克曼库尔特
- 美国
- 2088028
- 2025年07月16日
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文献和实验Preparation of nucleic acid probes
can also be used for labeling double-stranded DNA. In this case, fragments carrying label at one end only can then be generated by cleavage at an internal restriction site, generating two differently sized fragments which can be separated by gel electrophoresis
1) The ligation mixture contains the following: vector (~100 ng) insert (equimolar or 2 or 3 X molar concentration of vector) water added to 18 µl 2) Heat the mixture at 45 ℃ for 5 min. to melt any annealed cohesive ends. 3) Cool on ice. Add
LINEAGE ANALYSIS USING RETROVIRAL VECTORS
, if all of the cells that descend from a progenitor are the same type, the hypothesis is supported, but not proven, that the progenitor was committed to making only that cell type. In the latter case, a firm conclusion concerning the commitment of the progenitor
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