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文献和实验ensures a low background level of nonspecific probe adherence to the membrane and, second, an extended period of filter prehybridization is not required. The inclusion of a large amount of SDS does, however, necessitate that the nucleic acids
probe. In addition, the copy number of this sequence can be estimated under certain conditions. The different methods of application of material to filter give rise to the names of the different ways of blotting: dot blotting, slot blotting, and touch
Preparation of Fluorescent DNA Probe from mRNA: Yeast
filter by adding 5 uL ddH2O. Centrifuge 1 min. at top speed. Remove eluted water with pipet tip. 10. Add probe to filter. (Pipet probe onto filter wall, not directly onto membrane.)
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