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文献和实验Transformation of E. coli by Electroporation
for electroporation. 1. Use a fresh colony of DH5α (or other appropriate host strain) to inoculate 5 ml of SOB (without magnesium) medium in a 50 ml sterile conical tube. Grow cells with vigorous aeration overnight at 37EC. 2. Dilute 2.5 ml of cells into 250
CENTRIFUGAL FORCE Figure F.2. Nomogram for R.C.F. Modern day ultracentrifuges can generate forces in excess of 300,000 times that of gravity, forces sufficient to overcome the very cohesion of most molecules (including the metal of the rotor). The force
and other parameters will vary with the particular instrument and rotor used. Relative Centrifugal Force is a constant that is independent of the apparatus used. Figure F.2 presents a Nomogram for calculation of R.C.F. for a given radius and RPM. A simple formula
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