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文献和实验Procedure for Transfection of Mammalian Cells
cloudy, however this will not impede the transfection. Wash the cells once with 2 ml serum-free IMDM. For each transfection, add 750 μl serum-free IMDM to each tube containing the lipid-DNA complexes. Do not add antibacterial agents to media
for the technique of embedding specimens in plastic
for base layer Amount of chemical required for covering layer Pyrex glass dish 20cc or 20 ml Bio Plastic Catalyst 10 ml
How to Make Simple Solutions and Dilutions
(source solution attributes) V 1 C 1 =V 2 C 2 (new solution attributes) Example 1: Suppose you have 3 ml of a stock solution of 100 mg/ml ampicillin (= C1 ) and you want to make 200 ul (= C2 )of solution having 25 mg/ ml (= V2
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