Bandpass Filter, 610/30

Isolate DNA from NRBC Blood from Buccal Swab or dried blood spots collected with filter paper

mg/ml) into the sample. 2. Add 25ul OB Protease and 500ul Buffer BL into sample. Mix by vortexing at maxi speed for 30s. 3. Incubate at 65°C for 10 minutes. Collect any liquid drop from lid by brief centrifugation. 4. Centrifuge at 14

Hybridization of High Density Arrayed BAC Nylon Filter Blots

to keep wet. Place filter blots down on film (Kodak BioMax MS, 8326886) into X-Omatic Regular (Kodak) cassette with regular screens. Note: you may add special MS screens for increased sensitivity. Incubate -80°C, overnight. Leave cassettes

A rapid, quantitative and inexpensive method for detecting apoptosis by flow cytometry in transiently transfected cells

for eGFP, 610 nm longpass filter for PI, 675/20 nm bandpass filter for Cy5 and a 424/44 nm bandpass filter for DAPI. Doublets were excluded by pulse processing. Cells expressing eGFP were gated and analysed for either Cy5- or PI-fluorescence. Data