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文献和实验UPDATED DIRECTIONS FOR NEW BRUNSWICK MOBILE PILOT PLANT FERMENTOR
Hahn Lab Check manuals for more detailed information . Please Note: We have been having problems with the seal and bearing assembly at the bottom of the unit which allows the shaft to rotate inside the growth chamber.
胚胎和成年斑马鱼眼情的组织学准备Histological Preparation of Embryonic and Adult Zebrafish Eyes
as it is highly volatile; propylene oxide incubations and the subsequent steps should be performed in a hood until the samples are in embedding resin and transferred to an incubator (Step 9). Thaw embedding resin during the second propylene oxide
9 that induces DSBs to the target site, and the nickaseversion of Cas9 (Cas9n) bearing a deactivating mutation in one ofthe two nuclease domains, which nicks the target DNA on onestrand. For SpCas9 that we described in this protocol, the twoderived nickases
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