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文献和实验Direct Blotting Electrophoresis
them onto an immobilizing matrix that is moved across the bottom of the gel by a conveyor belt. DBE has been used for Southern blots, Western blots, DNA sequencing, and other applications (2 –11 ). Since this volume is dedicated to DNA sequencing, the following chapter
Rapid elution of DNA from agaro
columns. Materials 650 microliter Eppendorf tubes 1.7 ml Eppendorf tubes Sterile silanized glass wool Hypodermic needle and syringe Glass wool spin column preparation 1. Using a clean hypodermic needle, drive a hole in the end of a 650 microliter
Rapid elution of DNA from agarose gels
, drive a hole in the end of a 650 microliter Eppendorf tube from the inside then cut off the cap. 2. Wearing gloves, pull off a small piece of glass wool and roll it into a ball between hands. Place the glass wool plug in the bottom of the 650
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