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文献和实验Introduction Extraction and purification of plasmid DNA from E.coli can be performed on a small scale,where DNA is extracted from just 1-2 ml of bacterial culture (mini-prep), or from much larger volumes (100 ml %26ETH; maxi-prep; 1 L %26ETH
/Binding Buffer. Optional: For very small bead volumes (mini and micro isolations) use 50–100 μl wash volume to ease handling. 5) Proceed to section below. 3. Direct mRNA Isolation Protocol 1) Remove the Lysis
Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
) were fluorescently labeled when a small amount of plasmid DNA was pumped into the region. Scale bars: 250 µm in A , B , and C , and 50 µm in inset picture of C . vii. Remove the cathode and the embryo/insert assembly
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