- ￥10274
- Beckman Coulter贝克曼库尔特
- 美国
- C26742
- 2025年07月12日
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文献和实验Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.
E.Z.N.A.® HP Total RNA Kit Spin Protocol Eukaryotic Cells and Tissues
to the column. With the spin column inside a 2 ml collection tube (supplied with kit), centrifuge at 10,000 x g for 30-60 seconds min at room temperature. Discard flow-through and proceed to step 6. 6. Place column in a new 2 ml collection tube and add 300
was discarded, and the cell pellet was reconstituted in 0.5 ml PBS (with Ca2+ ), placed within accessory sample vials (Cat no. 383721, Beckman-Coulter, Fullerton, CA, USA) and analyzed for cell viability with the trypan blue exclusion assay, by utilizing
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