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文献和实验markers would place any pair of markers within 40 Kb of each other, on average. This level of physical linkage would result is the generation of markers that are _ 0.3 cM (0.3% recombination) within the mutant locus of interest. Thus AFLP should eliminate
Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
) Embryonic culture dish Prepare by pouring 3 mL of agar substrate into a 35-mm Petri dish (Fisher Scientific, 0875711YZ) (Chapman et al. 2001 ). Embryonic culture insert Prepare by pouring 1 mL of agar substrate into a Millicell
AFLP: not only for fingerprinting, but for positional cloning
frequencies over the entire genome (or equal distribution of markers throughout the genome), generation of 2500 markers would place any pair of markers within 40 Kb of each other, on average. This level of physical linkage would result is the generation
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