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- Beckman Coulter贝克曼库尔特
- 美国
- 363961
- 2025年07月13日
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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
the medium, and re-suspend the cells in an appropriate amount of fibroblast medium. d. Count the cells using the desired method (e.g., Countess® Automated Cell Counter), and seed the MEF culture dishes with 5 × 104 –2 × 105 cells per 100-mm dish
column into a clean 1.5 mL micro centrifuge tube. 10) Add 15-20 ul Elution Buffer(10mM Tris, pH 8.5) or water to the center of the membrane, incubate at room temperature for 3-5 minute. 11) Centrifuge at maximal speed ($13,000 x g
) for 1 minute to dry the column. 8) Place the column in a clean 1.5 mL microcentrifuge tube and add 10-20ul Elution Buffer (10mM Tris, pH8.5 ). Stand for 1-2 minute and centrifuge at maximal speed ($13,000 x g) for 1 minute to elute DNA.
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