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文献和实验为容积约 1升的扁平玻璃培养瓶。将液体或固体培养基在瓶中铺制成大面积的平面,用于大量培养细菌细胞。为 P. E. Roux命名。此外,通称茄形瓶。
Cell Thawing/Cell Freezing Protocol
/mL of cold freezing media Transfer 1mL of cells to appropriately labelled cryovials and maintain on ice for approximately 30minutes Transfer vials to -80C freezer for 24hrs Transfer to liquid nitrogen dewar or -140C freezer
Harvesting Hematopoietic Cells from Mice
Materials 4 mice from each genotype 4 Ly5 mice Buckets with wet ice 3x Bucket with dry ice 1x Dewar flask with liquid nitrogen 100 mL beakers with 95% ethanol 2x 100 mL beaker with dd. H2
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