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文献和实验Calcium Phosphate Transfection of PC12 Cells 磷酸钙法转染PC12细胞
).remove and save polylysine.wash plates 2x with 10ml sterile water.Seed cells: 1/2 X split from confluent plate (approximately 2-5 x 106 cells per 100mm plate) in PC12 culture media.Incubate overnight in 10% CO2 incubator.Cells should be 50-70% confluent
dT cellulose into a 2 ml Sarstedt screw cap tubes.2. Make fresh buffers: Note: Amounts are for 2 preps; scale up as necessary.♦ 2X NETS may need to be heated to go into solution.♦ 1X ETS should be heated to 65 ℃ for use.Protocol1. Resuspend
tip before adding to the buffer. Pump up and down several times to rinse out the inside surface. Mix in a fixative bottle, cap tightly, and warm in a water bath. 4. Cytoskeleton buffer with 1% glutaraldehyde, 30 ml. Mix in a fixative bottle, cap
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