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- Beckman Coulter贝克曼库尔特
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- 2025年07月12日
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文献和实验DROSOPHILA DNA PREP (essentially Roberts) 1. Grind 20-50 frozen flies with a mortar and pestle in N2(l) and suspend in 2 ml Lysis Buffer. 2.Add Proteinase K to 100 mg/ml; incubate 1-2h @ 37oC with occassional swirling.
Single Strand DNA Prep. for Sequencing
ampicillin 1 ml Q can be stored at 4℃ for several weeks Tetracycline Stock (1000X) 15 mg tetracycline 500 m l EtOH 500 m l Q vortex to dissolve and store at 4℃ Kanamycin Stock (1000X) 50 mg kanamycin 1 ml Q can be stored at 4℃ for several weeks 20
, take off supernatant, and let dry upside down 10 min. resuspend pellet in 50 ul TE buffer or water. You can now use 1-2 ul of this crude yeast plasmid DNA prep to transform E. coli. Materials breaking buffer 2% (v/v) Triton X
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