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文献和实验SDS-PAGE Western Blotting Protocols
). Part II – SDS-Polyacrylamide Gel Electrophoresis 1. For each SDS-PAGE gel, sandwich one small and one large glass plate,separated by spacers (smeared with silicone lubricant) and an alignment card. Optional: The inside surface of the small
Phosphoamino acid analysis:Mark Kamps's method
a -70℃.Film is much preferable to the phosphorimager because it is transparent and is exactly the same size as the plate and this facilitates alignment of the film and the plate.In the rare case that you need quantification,you can use the phosphorimager
Phosphoamino acid analysis:Mark Kamps method
to the phosphorimager because it is transparent and is exactly the same size as the plate and this facilitates alignment of the film and the plate. In the rare case that you need quantification, you can use the phosphorimager. 15. After developing the film, trace
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