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文献和实验Purification of Plasmid from 50 ml-culture
of cold 70% ethanol. Discard the fluid. 14. Add to the pellet 400 ul of TE containing DNase-free RNase A (20 ug/ml). Incubate the tube for 30 min at 37 C. 15. After 30 min, carefully check the content of the tube. If nucleic acid pellet
ES CELL DNA EXTRACTION: TUBE ME
off supernatant as in step 16. 19. Store the open tube on the bench at TRm until the last traces of fluid have evaporated. (i.e.: Air dry). 20. Dissolve the DNA in 30µl (or 50 µl, and use half of sample) of 10mM Tris pH 8.5 (or TE). (pipet
Fixation and permeabilization protocol 5/30/91 Mike Finney
from an unstarved plate with buffer. Wash them free of bacteria with water, leaving them in water in a microfuge tube on ice. - Add cold 2X MRWB to a final concentration of 1X and 20% formaldehyde to a final concentration of 1-4% (total volume usually 1 ml
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