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- Beckman Coulter贝克曼库尔特
- 美国
- 363664
- 2025年07月14日
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
1.5 ml 1.2 mM EDTA 0.5M stock 120 µl 1.1% Triton X-100 10% stock 5.5 ml 0.01% SDS 10% stock 50 µl
RUVKUN Antibody Staining Protocol revised 10/29/90
above (total volume= 5ml). Then add heptane(or water) and MeOH; EGTA. 100mM Tris Cl pH 7.4 1% Triton X-100 1 mM EDTA 1M H3BO3 0.5 M NaOH 0.1% BSA 1X PBS 0.5% Triton X-100 0.05% Na Azide 1mM EDTA
M sucrose in homogenization buffer containing 0.5 mM GTP,and pellet the membranes by centrifugation at 100,000 x g (29,000 rpm)for 1 h at 4℃ in a 50.2Ti rotor. 8.In the cold room,discard the supernatants and separately resuspend the dense,sticky ER
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