相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
相关阅读
细胞污染识别与处理全攻略:5 种常见类型+关键误区abinScience 体内级抗体,年度锁价,低至 1200五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海博耀商贸有限公司
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Preparation of BAC DNA with by Alkaline Prep Method
off supernatant and drain or wipe out excess supernatant. Should see pellet at this point. Dissolve the pellet in 700 µl of 50T/50E (50 mM Tris/50 mM EDTA, pH 8). Add 10 µl of RNase (10mg/ml) and leave at 37℃in a water bath for 1 hour. 12. Transfer the DNA
off supernatant and drain or wipe out excess supernatant. Should see pellet at this point. Dissolve the pellet in 700 µl of 50T/50E (50 mM Tris/50 mM EDTA, pH 8). Add 10 µl of RNase (10mg/ml) and leave at 37°C in a water bath for 1 hour. 11. Transfer the DNA
Protocols for ET recombination
temperature is important, usually 60℃ - 62℃ is optimal. Purify the PCR products by Qiagen column and elute with 2x 50 μl dH2O. Add 10x Dpn 1 buffer and 2 μl Dpn 1 (NEB) and digest (to eliminate template DNA) for 1 hour. Extract with Phenol:CHCl
技术资料暂无技术资料 索取技术资料






