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文献和实验全基因组的比较基因组杂交技术介绍(Whole-Genome and Custom Fine-Tiling Array CGH)
temperature (Tm = 76℃) across the entire set. Thus, probes are optimized to perform equivalently at a given stringency in the genomic regions your experiments require, including AT- and GC-rich regions.Custom Array CGHNimbleGen's highly flexible Maskless Array
fluorescence threshold can be set above the baseline. The parameter Ct (threshold cycle) is defined as the fractional cycle number at which the fluorescence passes the fixed threshold. So the higher the initial amount of the sample, the sooner accumulated
全基因组的比较基因组杂交技术介绍(Whole-Genome and Custom Fine-Tiling Array CGH)
, eliminating hybridization artifacts and/or bias and providing higher quality data. Probe lengths are adjusted (45mer - 85mer) to equalize the melting temperature (Tm = 76°C) across the entire set. Thus, probes are optimized to perform equivalently at a given
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