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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
Protease inhibitor cocktail for ChIP (1000X) Proteinase K (20 mg/mL) (Roche Diagnostics) Reagents for agarose gel electrophoresis RIPA buffer for ChIP RNase A SDS (sodium dodecyl sulfate) (10%) T cell isolation buffer
Midi/Maxi Kit. 16. Standard gel electrophoresis reagents and apparatus. 2.2 Cell Culture and Processing Reagents 1. Cell line: human embryonic kidney (HEK) 293FT cell line. 2. Dulbecco’s Modifi ed Eagle’s Medium (DMEM). 3. 10 % fetal
Fibroblast Cell Systems Instructions for Use
(CC-4081) 0.5 ml 10 ml FBS (Fetal Bovine Serum) (CC-4101) 3. ReagentPack TM (CC-5034) contains one 100 ml bottle of each of the following subculture reagents: HEPES Buffered Saline Solution (HEPES-BSS) (CC-5022)1 x 100 ml bottle Trypsin
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