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文献和实验Using Biacore to Measure the Binding Kinetics of an Antibody‐Antigen Interaction
Basic Protocol 1: Identification of Optimal Immobilization pH (pH Scouting) Basic Protocol 2: Immobilization of Ligand to Sensor Chip Using Amine Coupling Basic Protocol 3: Regeneration Scouting
Differential cDNA Screening Procedures
100 mM dTTP 8 ul 80 mM Na4P207 5 ul 4.0 mM (500 ug/ml) actinomycin D 100 ul 50 ug H20 597 ul Mix well and dispense in 100 ul aliquots and store at -70 C. 2.Incubate the reaction at 40 C for 1.0 hour. 3.Terminate reaction by placing on ice
SSR GEL and Silver Staining Protocol
Service Research 5985 Highway K Rhinelander, WI 54501 USA Phone: 715-362-1121 Fax: 715-362-1166 e-mail: pmarquar@newnorth.net I. EQUIPMENT: DNA sequencing unit (35 x 45 cm) & 2000V power supply Clamps Lg. plastic trays (4), about 43 x 50 x
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