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- Beckman Coulter贝克曼库尔特
- 美国
- C14280
- 2025年07月13日
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文献和实验Purification of Plasmid from 50 ml-culture
of cold 70% ethanol. Discard the fluid. 14. Add to the pellet 400 ul of TE containing DNase-free RNase A (20 ug/ml). Incubate the tube for 30 min at 37 C. 15. After 30 min, carefully check the content of the tube. If nucleic acid pellet
E.Z.N.A. Endo-Free BACs, PACs, P1s Protocol
with vigorous shaking (~ 300 rpm). Use a flask with a volume at least 4 times the volume of the culture. 2. Pellet 1.5-5 ml bacteria by centrifugation at 13,000 x g for 3 min at room temperature. Decant or aspirate medium and discard. 3. Resuspend
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
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