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文献和实验CGH Image acquisition Images were acquired through a Zeiss Axiophot fluorescence microscope using a Plan NEOFLUAR oil objective x63, N.A. 1.25 (Zeiss, Oberkochen, Germany) equipped with filter sets appropriate for DAPI (Zeiss filter set
Quantum Dots for Quantitative Flow Cytometry
is the quantum yield of the fluorophore, and %T is the percent fraction of emitted light that is transmitted by the bandpass filter at the detector PMT. The modular construction of beads decorated with discrete quantities of quantum dots with defined
(Track 1, Ch2) is the Cerulean intensity from resulting 458‐nm laser excitation collected through a 470‐ to 500‐nm bandpass filter. The un‐FRET panel (Track 1, Ch3) is the uncorrected FRET intensity resulting from 458‐nm laser excitation collected
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