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文献和实验Lacrimal Experimental Protocol (4/4/94)
other standards and unknowns. Make sure no fingerprints on capillary tube. Final DNA value is reading x 16 (4 x from instrument standardization; 4 x from dilution factor). 6. Combine data from A. and B. in Excel. Open a new worksheet. Type test substrates
) at the final concentration of 1 tablet/50ml): PBS Buffer A: 10 mM Hepes, pH 7.9, 10mM KCl, 1.5mM MgCl2, 0.5mM DTT S1 solution : 0.25 M Sucrose, 10 mM MgCl2 S2 solution: 0.35 M Sucrose, 0.5 mM MgCl2 S3 solution: 0.88 M Sucrose, 0.5 mM MgCl2
Medium Per Liter: To 950 ml of deionized H2 O , add: Tryptone 20 g Yeast Extract 5 g NaCl 0.5 g Shake until the solutes have dissolved. Add 10 ml of a 250 mM solution of KCl. (This solution is made by dissolving
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