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文献和实验). Staurosporine (800 ng/ml) was added following transfection as indicated (+ staurosporine). Excitation wavelength of 488 nm was used for eGFP and PI, 647 nm for Cy5 and multiline UV for DAPI. Emmision fluorescence was collected using a 530/20 nm bandpass filter
Isolation of Retroelement from Plant Genomic DNA
Primer 1 10 uM 5 50 nM.(50 pM total) Primer 2 10 uM
; 1000X), pH 8.0 (0.5 mM) 1.0ml - PIPES (150 mM; 10X), pH 7.4 (15 mM) pHM pre-Hybridization Mixture (make it fresh): 50% formamide ((Fluka) 4X SSC
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