- ￥2357
- Beckman Coulter贝克曼库尔特
- 美国
- A01-1-0049
- 2025年07月02日
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文献和实验The ribonuclease protection assay (RPA)
% acrylamide 9.31 mls of 2% bis acrylamide 7.45 mls of 10x TBE 35.82 g of Urea QS to 74.5 ml with dH2O 450 µl ammonium persulfate (10%) 60 µl TEMED Pour immediately into the gel mold, remove any air bubbles, and add an appropriate comb (e.g
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
corresponding to DNA hybridization signals can be determined using the x,y,z-measured distance module of the Metamorph software. The pixel size in your image can be calibrated with Metamorph (for example, the pixel size is 0.065 µm when using a 100X objective
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