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文献和实验E-Z 96® Viral RNA Protocol with Centrifugation
Buffer II diluted with ethanol to each well of HiBind® RNA plate. Seal the plate with a new sealing film. Centrifuge at 5500 x g for 5 minutes at room temperature. Note: Wash Buffer II Concentrate must be diluted with absolute ethanol before use
Mag-Bind® Plasmid Mega Magnetic Protocol
2. Tubes or vessel capable of 15,000 x g 3. 500mL centrifuge tube. 4. waterbath or heat block preset to 70℃ 5. Pipettor 实验步骤 1. Isolate a single colony from freshly streaked selective antibiotic plate and inoculate
Inducible shRNA Lentiviral Vectors
the remaining transformation mix at 4 °C. Plate out additional cells the next day, if desired. 3. Producing Lentivirus in 293FT Cells 1) Grow the 293FT Cells to obtain 6 x 106 293FT cells for each sample. 2) Prepare plasmid DNA
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