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- Beckman Coulter贝克曼库尔特
- 美国
- 6102051
- 2025年07月16日
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文献和实验Purification of acidic phosphatase from mustard seedlings
, and take a 1 ml sample labeled P1. Discard the remaining pellet . 8. Weigh the amount of solid (NH4 )2 SO4 required (0.25 g/ml of solution SN1). Record this amount. Leave the supernatant in a 250 ml beaker on ice bath and place on a magnetic stirrer
Purification of acidic phosphatase from mustard seedlings
the homogenate through 8 layers of cheese cloth into a cold beaker on ice. Remove 1 ml aliquot to an Eppendorf microfuge tube, and label as HOM and leave on ice. 5. Transfer the extract into one 250 ml centrifuge bottle. Balance against water or, if ready
Preparation of Embryonic Extract
bottle. 4. 2 sets of 100 mm sterile petri dishes (glass or plastic) on ice. 5. 1x 50 ml sterile beaker, keep on ice. 6. 3x 250 ml sterile beakers, keep on ice. 7. Cheese cloth, sterilized by autoclaving. 8. 250 ml Saline G, on ice
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