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文献和实验Real-Time Primer Design for DNA Chips
the corresponding position on the gene sequence. This prevents the gene sequence from swill during the washing process. A fluorescence marker is attached to the probe sequence so that the found sequence can be read out by a DNA chip reader. Figure 1. Sample spot
Dual- and Triple-Color Fluorospot
labeled with green fluorophore and SA labeled with Cy3, in the same tube to their respective working concentrations in PBS–0.1% BSA (seeNote 8). Add 100 μl/well and leave the plate for 1 h at room temperature. e. Wash the plate as in step
Taq-polymerase catalyzed cycle sequencing using fluorescent-labeled dye terminator reactions
to drain completely, by gravity. (Note: If flow does not begin immediately apply gentle pressure to the column with a pipet bulb.) 4. Insert the column into the wash tube provided. 5. Spin in a variable-speed microcentrifuge at 1300 g
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