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文献和实验Sepharose 4B-CL Chromatography BAC DNA Purification for Transgenic Production
, shake the tube gently to mix. Ideally, a genomic DNA:SDS:protein precipitate will form, and the remaining solution will be completely clear. Place on ice 15 minutes. 7. Spin at 7500 rpm in the SA-600 rotor for 15 min at 4C. Remove supernatant
in disodium EDTA containers and stored at -20ºC and a number of samples were also stored at -80ºC. To facilitate haemolysis of RBCs it is recommended to store a fresh sample for a few hours in a freezer as freezing destroys the red cells.
in disodium EDTA containers and stored at -20ºC and a number of samples were also stored at -80ºC. To facilitate haemolysis of RBCs it is recommended to store a fresh sample for a few hours in a freezer as freezing destroys the red cells.
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