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- Beckman Coulter贝克曼库尔特
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- 2025年07月11日
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文献和实验Single tube confirmation PCR protocol
Polymerase. - Add 35 µl of the PCR mix to each of the 20 PCR tubes that already contain the 15 µl of primers and template. 110 µl 5 µl 10 x Taq buffer(see below) 11 µl 0.5 µl 20 mM dNTP's (0.2 mM) 11 µl 0.5 µ
Purification of Plasmid from 50 ml-culture
the supernatant. Remove all of the fluid using pipetman. 10. Dissolve the pellet in 600 ul of TE. Transfer the solution into a microfuge tube. 11. Add 200 ul of 8M LiCl. Mix well, and then spin the solution at 14,000 rpm for 5 min at 4 C. 12
Purification of Plasmid from 50 ml-culture
. Discard the supernatant. Remove all of the fluid using pipetman. 10. Dissolve the pellet in 600 ul of TE. Transfer the solution into a microfuge tube. 11. Add 200 ul of 8M LiCl. Mix well, and then spin the solution at 14,000 rpm for 5 min
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