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- Beckman Coulter贝克曼库尔特
- 美国
- 363039
- 2025年07月14日
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文献和实验1 have swinging buckets. Since the buckets are easy to interchange, this type of rotor is extremely versatile. Its major drawback is the number of moving parts which are prone to failure with extended use. Nearly all cell biology laboratories
Preparation of Embryonic Extract
. 50.2 Ti rotor and tubes, chilled. 14. Millipore filters and holder: type AP, AW, and AA, 47 mm diameter. 15. Cameo 0.22 um filters (Fisher Scientific). 16. 50 ml syringe for applying pressure. 17. 3x 25 ml sterile flasks. 18. 15 ml
LABELING GIZZARD MYOSIN WITH IATR (MHC AND 17KD MLC)
(injection buffer). Procedure 1.Pellet myosin at 15,000 rpm,4℃,for 10 min in a SS34 rotor. 2.Soak and resuspend pellet in ~200μl of buffer 1.Handle gently. 3.Clarify at 25,000 rpm,4℃,for 20 min in a 42.2Ti rotor. 4.Determine myosin concentration using
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