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文献和实验全基因组的比较基因组杂交技术介绍(Whole-Genome and Custom Fine-Tiling Array CGH)
. Total features 385,000 Feature Size 16um x 16um Array size 17.4mm x 13mm Slide size 1" x 3" (25mm x 75
Replication timing by density transfer
, leu2-3,112, ura3-52, his6 in an A364A background. I aim for about 32 x 106 cells per time point. That gives enough DNA for at least 3 blots, leaving enough leeway for errors. 2. When the cell density is 2 x 106 cells/ml (OD660 ~ 0.16
for this protocol.)2. Wash the oligo dT cellulose 3X with 750 l 1X NETS. (Spin down gently between washes and aspirate off the wash.)3. Aspirate off final 1X NETS wash and resuspend the cellulose in 750 l 2X NETS.4. Incubate the RNA at 65 !C for 10 minutes.5. Add
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