- ￥1595
- Beckman Coulter贝克曼库尔特
- 美国
- FG0025031
- 2025年07月13日
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文献和实验Natural History Museum Protocol for EST sequencing from lamb
- (if the agarose gel in step 4 only shows a weak band, use less than 50µl of water to elute). Direct Purification Buffer: 50mM KCl (=0.37g/100ml) 10mM Tris-HCL (pH 8.8 at 25℃) (=0.12g/100ml) 1.5mM MgCl2 (=0.03g/100ml) 0.1% Triton X-100 (=100µl/100ml
E.Z.N.A.® HP Total RNA Kit Spin Protocol Eukaryotic Cells and Tissues
to the column. With the spin column inside a 2 ml collection tube (supplied with kit), centrifuge at 10,000 x g for 30-60 seconds min at room temperature. Discard flow-through and proceed to step 6. 6. Place column in a new 2 ml collection tube and add 300
Natural History Museum Protocol for EST sequencing from lambda zap phage plaques
through the column to wash the resin (discard the fluid that comes through) detach the syringe and place the column in a clean 1.5ml microtube. Spin for 30 seconds in microfuge at full speed (the kit protocol says to spin at 12,000G for 20 seconds
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