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文献和实验Question 1.What are the differences among RNase H, RNase A, RNase B and RNase C? 2.In your cDNA kits, RNase H is added in the second strand reaction to produce more nicked RNA as primers for DNA synthesis. In this repect
How to Maintain an RNase-free Lab
How to Maintain an RNase-free Lab Let's admit it, everyone who has worked with RNA has, at some point, been affected by RNase contamination. Sources of contaminating RNase include procedures that use RNase such as plasmid preps
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
reaction will require 160-300ng of template. 1. Streak clone stock to single colony on LB (+antibiotic) plate. Inoculate 1ml LB (+antibiotic) culture with single colony, grow overnight at 37?C. Using 3 ul of (overnight growth) single colony
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