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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
SDS lysis buffer/1x proteinase inhibitor cocktail (2.5 µl of 200 x stock from Upstate EZ ChIP kit). Roughly dissociate heads by pipetting, and transfer them into a 2 ml dounce homogenizer. Total volume is ~700 µl. �SDS lysis
国外蛋白组学专业BBS的一些问答-强烈推荐(11-14更新)
analysis? Thank you! A:This problem occurs very often. Better for cleaning and concentration of protein samples: Use the Ettan 2-D Cleanup Kit from Amersham Biosciences codegreen点评:这个问题由AMERSHAM著名科学家回答,比较会替公司做广告. 其实真核细胞总蛋白质的提取用不着使用TCA/acetone法,细胞相对于植物
risk as thecompleted reaction can be discarded immediately after processingwithout exposure to the laboratory environment. 4. Cleaning with 2–6% hypochlorite (w/v) has been shown todegrade DNA (4) (see Note 3). At this concentration, thelevels
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